Expert team, enhanced insights
Insights into metabolism
Our metabolomics analysis services can help you solve complex metabolic pathways, leading to significant breakthroughs in your project through biological research. We work towards making knowledge actionable for you through metabolomics.
Supporting research success
About us
We are Rubió Metabolomics, a preferred supply partner in the field of metabolomics.
Our commitment is to guide our clients from basic sample analysis to profound insights, making biological sense out of complex data with our comprehensive reports and powerful data analytics technologies. With our specialized expertise in metabolomic profiling, we support and accelerate your research journey, ensuring your success by addressing your scientific questions, research and diagnostic needs.
Comprehensive metabolomics services
Services
Our services feature specific UHPLC-MS analytical platforms, designed to adapt to your requirements and provide a wide view of the metabolome. Our metabolite coverage spans over 1300 compounds, including a diverse range of classes with varying physicochemical properties and concentration ranges. We can deliver broad coverage of lipids, amino acids, bile acids, and metabolites from the central carbon metabolic pathways. We evaluate proposals individually and can offer custom method development. Detailed data analysis, including correlation studies and predictive algorithms, is available upon request.
Lipidomics 1
Lipids represent an heterogenous and highly abundant metabolite class. The composition of the metabolome is highly dominated by lipids, particularly in human blood, where they constitute over 70% of detected metabolites.
Lipidomics 1 is an ultra-high performance liquid chromatography – Time of Flight – mass spectrometry (UHPLC-ToF-MS) based platform used for the analysis of sample extracts following a methanol extraction. This high throughput semiquantitative lipid profiling provides coverage over more than 300 lipids from different classes:
- Fatty acyls:
- Fatty acids (FA)
- Oxidized fatty acids (oxFA)
- Acylcarnitines (CAR)
- N-acyl ethanolamines (NAE)
- Glycerophospholipids (lyso):
- Glycerophosphocholines (LPC):
- monoacylglycerophosphocholines
- 1-alkylglycerophosphocholines
- 1Z-alkenylglycerophosphocholines
- Glycerophosphoethanolamines (LPE):
- monoacylglycerophosphoethanolamines
- 1-alkylglycerophosphoethanolamines
- 1Z-alkenylglycerophosphoethanolamines
- Glycerophosphoinositols (LPI):
- monoacylglycerophosphoinositols
- Glycerophosphoglycerols (LPG):
- Monoacylglycerophosphoglycerols
- Glycerophosphocholines (LPC):
- Sterol lipids:
- Bile acids (BA)
- Steroid sulfates (ST)
- Sphingolipids:
- Sphingoid bases (SPB)
Experimental results are greatly improved by optimal sample collection, processing, and storage. It is advisable to discuss the requirements with our technical staff.
Request a quote
Lipidomics 1
High-throughput semiquantitative lipid profiling based on specific methanol extraction and LC-MS analysis of more than 300 lipids of the following classes:
- Fatty acyls: non-esterified fatty acids, oxidized fatty acids, N-acyl ethanolamines and acylcarnitines.
- Sterol lipids: bile acids and steroid sulfates.
- Glycerophospholipids (lyso-forms): lysophosphatidylethanolamines, lysophosphatidylinositols, lysophosphatidylglycerols and lysophosphatidylcholines, including ether-linked species.
- Free sphingoid bases.
Learn more
Lipidomics 2
Lipids are more than energy storage entities and components of the biological membranes. As key regulators of systems’ homeostasis, lipids participate in metabolic regulation, cell signaling, proliferation or apoptosis, membrane trafficking, tissue physiology and can also interfere with therapy response.
Lipidomics 2 is an ultra-high performance liquid chromatography – Time of Flight – mass spectrometry (UHPLC-ToF-MS) based platform used for the analysis of sample extracts following a chloroform/methanol extraction. This high throughput semiquantitative lipid profiling provides coverage over more than 750 lipids from different classes:
- Fatty acyls:
- Primary fatty amides (FAA)
- Glycerolipids:
- Monoacylglycerols (MG)
- Diacylglycerols (DG)
- Triacylglycerols (TG)
- Glycerophospholipids:
- Glycerophosphocholines (PC):
- diacylglycerophosphocholines
- 1-alkyl,2-acylglycerophosphocholines
- 1-(1Z-alkenyl),2-acylglycerophosphocholines
- Glycerophosphoethanolamines (PE):
- diacylglycerophosphoethanolamines
- 1-alkyl,2-acylglycerophosphoethanolamines
- 1-(1Z-alkenyl),2-acylglycerophosphoethanolamines
- Glycerophosphoinositols (PI):
- diacylglycerophosphoinositols
- Glycerophosphocholines (PC):
- Sterol lipids:
- Cholesteryl esters (CE)
- Cholesterol and cholesteryl sulfate.
- Sphingolipids:
- Sphingomyelins (SM)
- Ceramides (Cer)
- Monohexosylceramides (HexCer)
- Sulfatides (SHexCer)
Experimental results are greatly improved by optimal sample collection, processing, and storage. It is advisable to discuss the requirements with our technical staff.
Request a quote
Lipidomics 2
High-throughput semiquantitative lipid profiling based on specific chloroform/methanol extraction and LC-MS analysis of more than 750 lipids of the following classes:
- Fatty acyls: primary fatty amides.
- Sterol lipids: Cholesterol esters, cholesterol and cholesteryl sulfate.
- Sphingolipids: ceramides, monohexosylceramides, sphingomyelins and sulfatides.
- Glycerolipids: monoacylglycerols, diacylglycerols and triacylglycerols.
- Glycerophospholipids: phosphatidylethanolamines, phosphatidylinositols, and phosphatidylcholines, including ether-linked species.
Learn more
Amino acids
Amino acids are the essential building blocks of proteins, critical for building and repairing muscle, tissues, and organs. However, their function is not limited to this role; amino acids are also a source of energy and regulate key metabolic pathways that are necessary for maintenance, growth, reproduction, and immunity.
Amino acids and their derivatives are analyzed in an ultra-high performance liquid chromatography – single quadrupole detector – mass spectrometry (UHPLC-SQD-MS) based platform used for the analysis of sample extracts following a methanol extraction and derivatization of primary and secondary amine groups. This analytical platform provides coverage over more than 40 amino acids and derivatives:
- The twenty canonical amino acids that comprise proteins:
- Essential amino acids: histidine (His), isoleucine (Ile), leucine (Leu), lysine (Lys), methionine (Met), phenylalanine (Phe), threonine (Thr), tryptophan (Trp) and valine (Val).
- Nonessential amino acids: alanine (Ala), arginine (Arg), asparagine (Asn), aspartate (Asp), cysteine (Cys), glutamine (Gln), glutamate (Glu), glycine (Gly), proline (Pro), serine (Ser) and tyrosine (Tyr).
- Di- and tripeptides, including GSH and GSSG.
- Alpha and Gamma amino acids derivatives.
Experimental results are greatly improved by optimal sample collection, processing, and storage. It is advisable to discuss the requirements with our technical staff.
Request a quote
Amino acids
This analytical platform provides coverage over more than 40 amino acids and derivatives:
- The twenty canonical amino acids that comprise proteins.
- Di- and tripeptides
- Alpha and Gamma amino acids derivatives.
Learn more
Expert team, enhanced insights
Why Rubió Metabolomics
Metabolomics is fast becoming a promising leading field in the practice of precision medicine. The close association between metabolites and phenotype can provide physicians and researchers with a powerful tool to obtain valuable, real-time information on the pathophysiological condition of an individual. Among other promising fields of study, metabolomics has proven useful to enable precise advancements in disease identification, characterization of disturbances, exploration of therapeutic targets, and discovery of biomarkers for diagnosis and treatment monitoring.
Within metabolomics, lipidomics is a crucial field for identifying disease-specific biomarkers and monitoring drug therapies. Through its focus on the diverse roles of lipids, lipidomics plays a pivotal role in disease diagnosis and prognosis.
Our Analytical Platforms
Rubió Metabolomics has optimized complete and highly productive metabolomic platforms. Our services include specific UHPLC-MS analytical platforms, tailored to your unique experimental needs and design. CTA: Read more about our Metabolomic services.
The Expertise of Our Team
The strength of Rubió Metabolomics lies in our multidisciplinary Scientific Team. With a fusion of scientific expertise, open communication and trust, our team creates enduring connections. We offer scientific guidance from experts in the field, and effective counsel for optimal experimental design from sample collection to statistical discussion.
Above and Beyond the Bare Essentials
Rubió Metabolomics is more than just a service provider. According to our clients, our metabolomics specialists go above and beyond the essentials to make your experience fulfilling. We provide added value to your research by simplifying the interpretation of complex data, helping you to turn raw information into actionable metabolic insights.
Excellence in esearch
How we work
At Rubió Metabolomics, we are committed to delivering top-quality services for cutting-edge research. Our approach involves strict control over analytical procedures and the utilization of state-of-the-art facilities. We have a professional team of experts in the field ready to accommodate your needs. Here’s an overview of how we work:
1. Initial Contact and
Experimental Planning:
Our qualified team of bioanalytical chemists gets in contact with you to discuss project details and provide technical guidance. We help design optimal experiments, including sample collection, preparation, analysis, and data processing, aiming for high-quality, fast, and cost-efficient results.
2. Metabolite Extraction and LC/MS Analysis:
Sample prep is crucial in metabolomics, impacting metabolome coverage. We use specific methods based on the analysis requested, tailored to the sample type and metabolome portion of interest (e.g. lipids, amino acids).
Our platforms include liquid chromatography coupled with mass spectrometers (UPLC-MS), that offer selective and sensitive analyses for metabolite identification. Samples are analyzed in positive/negative ESI modes, scanning from m/z 50-1200 on a reverse-phase column chosen based on the application. Rubió Metabolomics counts on both high- and low-resolution mass spectrometers that are used according to the analysis requirements.
3. Data Analysis:
Data handling is a challenging and critical step in metabolomics, requiring expertise in bioinformatics, statistics, and specialized software.
At Rubió Metabolomics, we utilize powerful informatics and statistical applications to manage metabolomics datasets. Our analytical platforms are supported by state-of-the-art software and visualization tools, enabling transformation of complex data into informative charts and graphs. We employ chemometric tools like PCA, PLS-DA, OPLS, and univariate statistical analysis.
4. Report Writing:
Our results package includes a detailed report of experimental methods, including a description of the results obtained from the data analysis and interpretation of these results. We also provide the obtained metabolomics data set.
Proven record
Track
Record
Since 2006 we have established a strong track record in the field of metabolomics. Throughout our journey, we have successfully completed more than 350 service projects. Through these years we have led and been involved in a great number of R&D projects in a continuous dedication to advance scientific knowledge, contributing to the monitoring and diagnosis of human and animal health. Our work is endorsed by a great number of peer-reviewed scientific articles published.
Verified excellence
Certifications
We take great pride in the extensive recognition and certification from numerous health and governmental institutions we have received over the years. These certifications are a proof of our commitment to quality, innovation, and excellence in the field of metabolic sciences.
